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. 2010 Mar 31;2(2):91–104. doi: 10.3390/pharmaceutics2020091

Table 2.

Selected proteins and their physical characteristics with relation to 125I-radiolabelling.

Protein Size (kDa) pI No Tyrosine residues 125I-labelling Buffer/pH 125I-labelling efficiency % adsorption to DDA:TDB liposomes
OVA 45 4.5 9 Tris/7.4 +++ 88 %a
BSA 66 4.7 21 Tris/7.4 +++ N/D
Lysozyme 14.7 11 3 Tris/4 ++ 29 %b, [22]
Ag85B-ESAT-6 44 4.9 13 Tris/7.4 +++ 97 %b, [22]

a Determined using the BCA assay; b determined using 125I-radiolabelling; N/D, not determined.