Table 4.
Location of AFLP sequences, list of primers and restriction enzymes for genotyping of SNP markers
| Markers | Chromosome | Forward primer | Reverse primer | Annealing Tem. (°C) | Enzyme |
|---|---|---|---|---|---|
| AFLP01 |
2 |
5'-tgtatttccaacctactctac-3' |
5'-gctgtacgtaaggctgcag-3' |
58 |
TaqI |
| AFLP02 |
3 |
5'-gcacctggaagaatagatag-3' |
5'-gtctttcctggtattcttatg-3' |
56 |
EcoRI |
| AFLP03 |
4 |
5'-caggaacagcatagaattaaag-3' |
5'-caccagtgaccaaggcaag-3' |
58 |
TaqI |
| AFLP04 |
15 |
5'-agctgctctcttcagtcag-3' |
5'-agatttcaaagtgtcatgtgc-3' |
58 |
TaqI |
| AFLP05 |
4 |
5'-tgcaatgcttatctacctgg-3' |
5'-cgtctatctggaaattgctg-3' |
58 |
TaqI |
| AFLP06 |
11 |
5'-gagaacctgctggtggtg-3' |
5'-tccatgtggcggacgatg-3' |
60 |
Hsp92II |
| AFLP07 |
4 |
5'-gtttcagcaaggcagagttc-3' |
5'-gaccaaacaacgtcacgttc-3' |
58 |
TaqI |
| AFLP08 |
6 |
5'-tttgtttcagcaaggcagag-3' |
5'-gaccaaacaacgtcacgttc-3' |
58 |
TaqI |
| AFLP09 |
1 |
5'-aaccatgcagccagagaatg-3' |
5'-gcagccattcatacatggtc-3' |
58 |
EcoRI |
| AFLP10 |
14 |
5'-gctctgatcaaagacattcc-3' |
5'-gatcatcttcggctacagag-3' |
58 |
AluI |
| AFLP11 |
11 |
5'-gtgcagctggggttggg-3' |
5'-ggaaccggtgcttgcattg-3' |
58 |
BsuRI |
| AFLP12 |
1 |
5'-tgaccatgctttctccatag-3' |
5'-tttggaaatcaattttcagctc-3' |
58 |
TaqI |
| AFLP13 |
1 |
5'-gcagctgcgtataaacacag-3' |
5'-caggactgcagggataaatg-3' |
58 |
TaqI |
| AFLP14 |
10 |
5'-gcttcagcaggcagatttc-3' |
5'-ctttacgtggcccaccttc-3' |
58 |
BsuRI |
| AFLP15 |
2 |
5'-aaactcttttcccttggctg-3' |
5'-gtgcaagacggactgtattg-3' |
58 |
EcoRI |
| AFLP16 |
8 |
5'-actcgcaggagaacttcttag-3' |
5'-gcagtcctgtgacttatttg-3' |
58 |
EcoRI |
| AFLP17 |
1 |
5'-gaaatcgttgccaaaagttgc-3' |
5'-gttcacgcagctcggatg-3' |
58 |
MspI |
| AFLP18 |
7 |
5'-ggtttgatttctgggatctc-3' |
5'-gccttaggtaacattccttc-3' |
58 |
EcoRI |
| AFLP19 |
2 |
5'-cctcctactgattctgtaatg-3' |
5'-ttttctgctcatctgtactgg-3' |
56 |
Hin6I |
| AFLP20 |
4 |
5'-aggatcacaaataaccaacga-3' |
5'-gactacagtgagaagctctg-3' |
60 |
MboI |
| AFLP21 |
10 |
5'-gtctgcacacctggtgtc-3' |
5'-caggttcacacggagatc-3' |
58 |
MspI |
| AFLP22 |
1 |
5'-ggaggttcgtgagaagctg-3' |
5'-tgtacaacagcagcaagcaaa-3' |
60 |
Hsp92II |
| AFLP23 |
8 |
5'-ctttctccttctccccaagt-3' |
5'-cttggatagggtctgcaga-3' |
58 |
TaqI |
| AFLP24 |
1 |
5'-gcagtgcacctggattttag-3' |
5'-attatcccttccctcagctg-3' |
60 |
TaqI |
| AFLP25 |
17 |
5'-ctgctagcaggtaatgagat-3' |
5'-tggcagaaagattccgtcaa-3' |
55 |
TaqI |
| AFLP26 |
2 |
5'-tctctgctagtgtgtgtgga-3' |
5'-caccaggactgaagaacaga-3' |
60 |
EcoRI |
| AFLP27 |
3 |
5'-ctgagaatagccaggacaca-3' |
5'-gtgttgggaatttaggaaac-3' |
58 |
TaqI |
| AFLP28 |
11 |
5'-gtgagggcaaccagagcca-3' |
5'-tgaagattcctgttcttgag-3' |
58 |
Hsp92II |
| AFLP29 |
9 |
5'-ctgctgcggaccgaaatatc-3' |
5'-gatagttccgaacagtttgc-3' |
58 |
BsuRI |
| AFLP30 | 13 | 5'-cagatgatcacagtaacctg-3' | 5'-gtggaacttgtaagtacgc-3' | 58 | TaqI |