Table 1.
ECM | Stock concentration | Final concentration | Source |
---|---|---|---|
Poly-D-lysine |
1 mg/ml |
50 μg/ml |
Sigma P7280 |
Type I collagen |
2.5 mg/ml |
100 μg/ml |
Sigma C9791 |
Fibronectin |
1 mg/ml |
20 μg/ml |
Sigma F1141 |
Vitronectin |
50 μg/ml |
2 μg/ml |
Sigma V8379 |
Laminin | 0.9 mg/ml | 10 μg/ml | Invitrogen 23017-015 |
ECM proteins were added at the indicated final concentration in a volume of 500 μl, and left overnight at 4°C.