Figure 1.

Alignment and phylogenic tree of the C-terminus of PC2. A) Alignment of EF hands from PC2 orthologs, including hPC2 and suPC2. Sequence alignments were calculated in Clustal Omega using BLAST search results from the cytoplasmic tail of hPC2 and analyzed in JalView. B) Phylogenic tree of the C-terminal tail of PC2. Note that PC2 orthologs from chordates and nonchordates fall into either groups containing 2 Ca²⁺ sites or 1 Ca²⁺ site. The C terminus of suPC2 was used an input sequence under a BLAST-explorer search to find similar proteins using Phylogeny.fr (36). Example sequences across different species were then selected and aligned using Clustal Omega. The phylogeny tree was then constructed using Pylogeny.fr (37). Numbers refer to the amino acids chosen for analysis.