Figure 2.

DCG (LDCV) area but not number is decreased in adrenal medullary chromaffin cells from VGF-knockout (Vgf^−/−) compared to wild-type (Vgf^+/+) mice. A–D) Representative EM micrographs of Vgf^+/+ (A, C) and Vgf^−/− (B, D) adrenal medullary chromaffin cells, taken at ×10,000 (A, B) and at ×30,000 (C, D). Scale bars = 2 μm (A, B); 500 nm (C, D). E–G) Tissues were prepared for EM, and ×30,000 images were analyzed using MetaMorph to determine mean LDCV core area in square nanometers (E), LDCV number per square micrometer of cytoplasm (F), and the distribution of LDCVs having specified square nanometer areas (G). Values are means ± sem. LDCV core area in Vgf^+/+ chromaffin cells was significantly different from that in Vgf^−/− cells (n=3228 Vgf^+/+ DCVs; n=2551 Vgf^−/− DCVs). ***P < 0.0001; 2-tailed Student's t test. H–K) As shown (H), the area around the dense core (J) and the area of the membrane-bound LDCV (K) were both significantly decreased in Vgf^−/− chromaffin cells (I). *P < 0.05, **P < 0.005; unpaired 2-tailed Student's t test.