Figure 3.

Adrenal CgB protein levels are reduced in Vgf^+/− and Vgf^−/− mice in comparison to wild-type Vgf^+/+ mice. Adrenal glands were isolated from wild-type Vgf^+/+, heterozygous-knockout Vgf^+/−, and homozygous-knockout Vgf^−/− mice and extracted in RIPA buffer, and Western blot analysis for CgA, CgB, SgII, and SgIII was carried out. Levels of CgA (A), CgB (B), SgII (C), and SgIII (D) were determined by densitometry of scanned films (E) and normalized to their respective actin loading controls, and are expressed relative to the level of the respective wild-type granin (means±sem; n=2–5 mice/group). *P < 0.05, **P < 0.005; ANOVA with Tukey's post hoc comparison.