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. 2014 Mar 28;10:75. doi: 10.1186/1746-6148-10-75

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Copyright © 2014 Chai et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Development of systemic and mucosal immune responses after TGEV infection. (A) TGEV-specific antibodies measured by competitive ELISA are shown as optical densities (OD), measured at 450 nm, from 0 to 18 dpi. An OD₄₅₀ value higher than 0.9 was considered negative (upper dashed line), an OD₄₅₀ value lower than 0.77 was considered positive (lower dashed line), and an OD₄₅₀ value between 0.9 and 0.77 was considered questionable. (B) The concentration of sIgA in intestinal wash fluid was measured by a direct ELISA. Results are shown as the mean value ± standard deviation. Asterisk indicates statistically significant difference (P ≤ 0.05) between the groups.