Table 1.
Cue treatment |
N |
Rationale |
Classification |
||
---|---|---|---|---|---|
Treatment | Lysed parasites | Lysed RBC | |||
Control |
5 |
No-treatment control for the stress of handling and injections |
C |
NP |
NL |
Uninfected RBC |
5 |
Control for the stress of handling and injecting the host with blood |
U |
NP |
NL |
Uninfected lysed RBC |
10 |
To test for a response to RBC debris |
UL |
NP |
L |
AJ-infected lysed RBC |
10 |
Compare AJ to UL to test for a response to high density of self |
AJ |
P |
L |
ER-infected lysed RBC | 10 | Compare ER to AJ to test for a response to non-self | ER | P | L |
The analysis involved comparing individual cue treatments and comparing treatments grouped in different ways to test whether parasites respond to lysed parasite material (P vs NP) and/or to lysed RBC material (L vs NL) . N = number of mice that received a particular treatment.