. 2014 Mar 26;13:115. doi: 10.1186/1475-2875-13-115

Table 2.

Summary of ANOVA analyses

	Cohort 1		Cohort 2
	Prior: day 4	Post: days 5-7	Prior: day 10	Post: days 11-13
Asexual density	F_{4, 34} = 1.13, p = 0.36	F_{4, 34} = 0.79, p =0.54	F_{4, 25} = 0.59, p = 0.68	F_{4, 24} = 0.14, p = 0.97
RBC density	F_{4, 34} = 1.00, p = 0.42	F_{4, 34} = 1.70, p = 0.17	F_{4, 28} = 1.62, p = 0.20	F_{4, 24} = 0.45, p = 0.77
Reticulocyte proportion	F_{4, 34} = 1.05, p = 0.40	F_{4, 34} = 0.32, p =0.86	F_{4, 28} = 0.77, p = 0.56	F_{4, 24} = 1.53, p = 0.23
Gametocyte density	F_{4, 34} = 0.17, p = 0.95	F_{4, 34} = 0.39, p = 0.81	F_{4, 28} = 1.60, p = 0.20	F_{4, 20} = 1.73, p = 0.18
Sex ratio	F_{4, 31} = 1.27, p = 0.30	F_{4, 34} = 0.60, p = 0.67	F_{4, 28} = 0.63, p = 0.64	F_{4, 26} = 0.22, p = 0.93

Asexual density and the in-host environmental parameters of RBC density and proportion of reticulocytes did not vary significantly across the treatment groups - either prior to, or post cue administration, in either cohort. Furthermore, gametocyte density and sex ratio did not vary significantly prior to cue administration. This means that the effects of the cue treatments were not confounded by unintended variation in the in-host environment or pre-existing variation in gametocyte density and sex ratio (see also Additional file 1: Figure S1).