Table 1.
Bovine specific oligonucleotide forward and reverse primer sequences (5′-3′) and PCR product length
| Gene name | Sequence | Accession number | Amplicon size (bp) |
|---|---|---|---|
| 18S/28S | F: 5′- TGCTCTCGCAAACCTAACCT-3′ | DQ222453 | 159 |
| R: 5′- CACTAAGCACTCGCATTCCA-3′ | |||
| ACTA2 | F: 5′- ACTGGGACGACATGGAAAAG -3′ | BT021508 | 166 |
| R: 5′- TACATGGCTGGGACATTGAA-3′ | |||
| ACTB | F: 5′- ACTTGCGCAGAAAACGAGAT-3′ | BT030480 | 121 |
| R: 5′-CACCTTCACCGTTCCAGTTT-3′ | |||
| APEH | F: 5′- CAAGAGCATGCGCAGTATGT -3′ | BC123400 | 181 |
| R: 5′- GTAGAGCTGCAAAGCCCATC-3′ | |||
| CELA1 | F: 5′- GGAACCATCCTGGCTAACAA -3′ | BC149525 | 165 |
| R: 5′- CATGGTGGTCTTCACAGTGG -3′ | |||
| DONSON | F: 5′- TGTGTTGGTGAAGGGAATGA -3′ | BC133573.1 | 107 |
| R: 5′- AGAGGGTTGGTGGAAGTCCT -3′ | |||
| FST | F: 5′- TAAATGAGAGACCCGCCAAC-3′ | AY775795 | 171 |
| R: 5′- CCCCAGTTTCTGTCCTGTGT-3′ | |||
| GALNT6 | F: 5′- GACCACGTCTTGGACCTCAT-3′ | NM_001015534 | 146 |
| R: 5′- AGCTCAGCTGGGGTGTAGAA-3′ | |||
| GAPDH | F: 5′- GGGTCATCATCTCTGCACCT-3′ | NM_001034034 | 176 |
| R: 5′- GGTCATAAGTCCCTCCACGA-3′ | |||
| GJA1 | F: 5′- - CAACATGGGTGACTGGAGTG 3′ | BT021508 | 110 |
| R: 5′- GCAGGATTCGGAAAATGAAA -3′ | |||
| IL33 | F: 5′- TTGTTTTGGAGGATGGAAGC -3′ | BC123562 | 163 |
| R: 5′- TTTGTGGGGCTCAGGTTTAC -3′ | |||
| MMP19 | F: 5′- TGGACGTTATCCCCTCAGTC-3′ | BC123722 | 119 |
| R: 5′- GTCCATGGTTCATGCTTGTG -3′ | |||
| MOSC2 | F: 5′- GCAGTGCTTTTGAGGAGGAC-3′ | NM_001076380 | 169 |
| R: 5′- GGATCACACAGGCGGTAACT-3′ | |||
| NMB | F: 5′- ACATGACGACATGGCTGAAA-3′ | NM_001075270 | 185 |
| R: 5′- ACTTCAACAGGGAAGCGAGA-3′ | |||
| NPPC | F: 5′- GAGGCAACAAGAAGGGTTTG -3′ | BC123399 | 149 |
| R: 5′- CTGATGACCAAGGGTGACCT -3′ | |||
| PPARA | F: 5′- TTGTGGCTGCTATCATTTGC-3′ | AF229356 | 135 |
| R: 5′- AGAGGAAGACGTCGTCAGGA-3′ | |||
| RAB3B | F: 5′- TGGGCGGAGATTCATTTTAC -3′ | BC112795 | 144 |
| R: 5′- GAAAAGTGTGCATGGGTGTG -3′ | |||
| RPL15 | F: 5′- TGCATAAGCACAGGGAGATG-3′ | BT020706 | 134 |
| R: 5′-CTGGAGAGTATTGCGCCTTC-3′ | |||
| SFRP1 | F: 5′- GTCCCTCTGGGTGAATCTGA-3′ | NM_174460 | 158 |
| R: 5′- TCACTAATTGCCAGGGGTTC-3′ | |||
| SLC1A3 | F: 5′-CATCCATGCTGTCATTGTCC-3′ | BC120125 | 188 |
| R: 5′-ATCTGGTAACGCGTTTGTCC -3′ | |||
| SLC45A2 | F: 5′- CATGCCCTCTTCACAGGTTT-3′ | XM_001251343 | 179 |
| R: 5′- AGTGGGGCTTCAGGGATACT -3′ | |||
| TGFB1I1 | F: 5′- CCTGCAATAAACCCATTGCT-3′ | NM_001035313 | 162 |
| R: 5′- AGAAGCGCTCGAAGTAGCAC-3′ | |||
| UBQ | F: 5′- TACAACAGTTGGTGGCCAAA-3′ | BC102888 | 121 |
| R: 5′-GAAGACTGGGCTGACTGAGG-3′ |
18S/28S, 18S/28S ribosomal RNA; ACTA2, actin alpha 2; ACTB, beta-actin; APEH, N-acylaminoacyl-peptide Hydrolase; CELA1, chymotrypsin-like elastase family member 1; DONSON, downstream neighbor of SON; FST, follistatin; GALNT6, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 6; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GJA1, gap junction protein, alpha 1; IL33, interleukin 33; MMP19, matrix metallopeptidase 19; MOSC2, MOCO sulphurase C-terminal domain containing 2; NMB, neuromedin B; NPPC, natriuretic peptide C; PPARA, peroxisome proliferator-activated receptor alpha; RAB3B, RAB3B member ras oncogene family; RPL15, ribosomal protein L15; SFRP1, secreted frizzled-related protein 1; SLC1A3, solute carrier family 1 member 3; SLC45A2, solute carrier family 45 member 2; TGFB1I1, transforming growth factor beta 1 induced transcript 1; UBQ, ubiquitin.