Table 2.
Efficiency variables for individual RT-qPCR genes
| Gene | Optimum [primer] μM | Slope (−) | R 2 | Efficiency |
|---|---|---|---|---|
| ACTA2 | 5 | 3.62 | 0.99 | 1.890 |
| APEH | 20 | 3.18 | 0.99 | 2.064 |
| CELA1 | 10 | 3.54 | 0.99 | 1.917 |
| DONSON | 10 | 3.20 | 0.99 | 2.054 |
| FST | 2.5 | 3.60 | 0.97 | 1.896 |
| GALNT6 | 5 | 3.29 | 0.99 | 2.103 |
| GJA1 | 20 | 3.31 | 0.98 | 2.003 |
| IL33 | 5 | 3.33 | 0.96 | 1.997 |
| MMP19 | 10 | 3.52 | 0.99 | 1.925 |
| MOSC2 | 10 | 3.14 | 0.99 | 2.082 |
| NMB | 10 | 3.21 | 0.99 | 2.049 |
| NPPC | 5 | 3.63 | 0.99 | 1.885 |
| PPARA | 5 | 3.33 | 0.99 | 1.997 |
| RAB3B | 20 | 3.51 | 0.99 | 1.927 |
| SFRP1 | 5 | 3.70 | 0.97 | 1.863 |
| SLC1A3 | 10 | 3.46 | 0.97 | 1.946 |
| SLC45A2 | 10 | 3.54 | 0.95 | 1.916 |
| TGFB1I1 | 20 | 3.29 | 0.96 | 2.013 |
| UBQ | 2.5 | 3.20 | 0.99 | 2.054 |