Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Mar 26;11:58. doi: 10.1186/1742-2094-11-58

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Vilalta and Brown; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Deoxyglucose rapidly depletes ATP prior to microglial death. (A) BV-2 density at different time points (0, 1 hour, 3 hours, 6 hours, 24 hours, 48 hours) after addition of 10 mM deoxyglucose (DOG) measured by flow cytometry. (B) Cellular ATP levels determined with luciferin/luciferase at the same time points. RLU, relative light units. (C) Percentage of Annexin V-positive BV-2 cells measured by flow cytometry at the same time points. (D) Percentage of propidium iodide (PI)-positive BV-2 cells measured by flow cytometry at the same time points. Data presented as mean ± standard error of the mean for ≥ 3 independent experiments.*P < 0.05, ***P < 0.001, ****P < 0.0001.