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. 2014 Mar 25;14:77. doi: 10.1186/1471-2229-14-77

Table 9.

Non-destructive plant variables measured in seedlings under greenhouse conditions

Trait Abbreviation Date of measurement Description
Days to germination (d)
D_G
2-4 weeks after sowing
From day of sowing, number of days until appearance of shoot from soil.
Days to cotyledon emergence (d)
D_CE
2-4 weeks after sowing
From day of sowing, number of days until both cotyledons were fully open.
Number of cotyledons (#)
N_Cot
2-4 weeks after sowing
Number of cotyledons per plant.
Plant height (cm.)
P_H
30 DAG
Plant height from the plant base at the soil to its apex.
Stem diameter (cm.)
S_D
30 DAG
Stem diameter at the base of the trunk.
First leaf length (cm.)
FL_l
30 DAG
For the first emerged leaf, length was measured from the point at which the petiole joins the leaf, to the tip of the leaf.
First leaf width (cm.)
FL_W
30 DAG
For the first emerged leaf, width was measured between the two furthest horizontal (and parallel) points across the leaf.
Total number of leaves (#) Nr_L 60 DAG Overall number of mature leaves, excluding cotyledons and emerging leaves around the plant’s apex.

Sowing date was December 20, 2008. DAG = days after germination.