Figure 3.

(A and B) VEGF and TGFβ1 pro-angiogenic factors accumulation were analyzed by immunoblotting, followed by densitometric analyses of the blots using ImageJ. Each bar represents the mean ± SE of the values obtained (n represented within the figure). (C and D) Conditioned media from bone marrow-derived macrophages from WT or KO cells and Tm1N3 mock-cells or Tm1G3 galectin-3 transfected cells after 24 h of culture, dosed by ELISA of secreted VEGF to media (wells)/experiment (n = 6) in basal line (0) and after TGFβ1 stimuli (50 ng/mL, 100 ng/mL). The results were submitted to unpaired test, two-tailed with *P < 0.1; ** P < 0.01 and ***P < 0.001. Each bar represents the mean ± SE and the graph is a representative result from four independent experiments. WT, wild type; KO, knockout; ELISA, enzyme-linked immunosorbent assay.