Table 1.
Genes differentially induced by TM between control and Lrh-1-null mice and their regulation by ATF2 and LRH-1
| Gene | Known target of | |
|---|---|---|
| ATF2 | LRH-1 | |
| PLK3 | ✓ | ✓ |
| GDF15 | ||
| FGF21 | * | |
| PMM1 | ||
| CRELD2 | ✓ | |
| NFIL3 | ||
| IGSF11 | ||
| GSTM2 | ||
| GOT1 | ||
| DPP9 | ✓ | ✓ |
| ST5 | ||
| CCBL1 | ✓ | ✓ |
| TMEM66 | ✓ | |
| KRTCAP2 | ✓ | ✓ |
| LRRC59 | ✓ | ✓ |
| CDK2AP2 | ✓ | ✓ |
| ST3GAL1 | ||
| TES | ✓ | |
| CCDC134 | ✓ | ✓ |
| UGT1L | ||
| ACOT2 | ✓ | |
| PLIN5 | ✓ | |
| LITAF | ✓ | |
| RPS13 | ✓ | |
| LGALS3BP | ||
| CRYM | ||
| SUPT5H | ✓ | |
| B3GAT3 | ✓ | |
| GRN | ✓ | |
| ARRDC4 | ||
| SLC30A7 | ✓ | |
| CRELD1 | ✓ | |
| NT5M | ||
| ARSG | ||
| SEP15 | ||
| MKNK1 | ✓ | |
| DDX52 | ||
| EXOSC5 | ✓ | |
| RABAC1 | ✓ | |
| RPS15 | ✓ | ✓ |
| D830014E11RIK | ||
| AVPI1 | ✓ | |
| RPS9 | ✓ | |
| CRAT | ✓ | |
| BHMT2 | ✓ | |
| B230217C12RIK | ||
| EIF3G | ✓ | ✓ |
| SLC25A28 | ✓ | |
| HR | ||
| CCL9 | ||
| ANXA4 | ✓ | ✓ |
| SMCO4 | ||
| SMOX | ||
| ARL14EP | ✓ | |
| SLC39A7 | ✓ | |
| ICA1 | ||
| ENTPD5 | ✓ | |
| PIWIL2 | ||
| ANG | ✓ | |
| MCFD2 | ✓ | ✓ |
| SOAT2 | ||
| SLC41A3 | ✓ | ✓ |
| MFGE8 | ||
| CYP4A14 | ||
| D12ERTD647E | ||
Microarray analysis was performed for control and Lrh-1LKO mice treated with vehicle or 1 mg/kg tunicamycin for 24 hr (n = 3). Genes were screened for those induced at least 1.5 fold by TM in control mice with significantly different induction in Lrh-1LKO mice by t-test (p<0.05). This list was filtered for those with differential expression between genotypes with TM treatment by t-test (p<0.05). Previously published genome-wide ATF2 and LRH-1 binding datasets were analyzed to identify genes with ATF2 or LRH-1 binding sites −500 to +275 bp from the TSS. Genes in our set were compared with these sets and genes that contain ATF2 or LRH-1 binding sites meeting the above criteria are marked.
No binding site for ENCODE data set but a known ATF2 direct target (Hondares et al., 2011).