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. 2014 Mar 26;15:12. doi: 10.1186/1471-2121-15-12

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Copyright © 2014 Brandl et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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Angiogenic potence in Matrigel and fibrin glue of positively sorted primary cells is equal to an endothelial cell line. Positively sorted cells, negatively sorted cells and the liver endothelial cell line EC52 (pos. control) were seeded in cell culture flasks and their phenotype was investigated with light microscopy (top row). 5x10⁴ cells of each population were seeded on Matrigel™ (2^nd row) or resuspended in Matrigel™ (3^rd row) and 1x10⁵ cells of each population were suspended in fibrin glue (bottom row) to investigate their performance in tube formation assays. Microscope pictures were taken 24 h after cell seeding. The scale bar depicts 100 μm.