Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Apr 14;205(1):83–96. doi: 10.1083/jcb.201311104

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 2. — SIM can resolve actin arcs on the dorsal surface of the lamella. (A–E) 3D SIM of actin filaments in a U2OS cell. (A) Side view from the region of interest denoted by the box in B. Red dots show orientation. The bottom focal plane (purple) is similar to a total internal reflection fluorescence (TIRF) microscopy image. Subsequent 2.2-µm sections above the bottom focal plane are labeled blue, green, and orange, respectively. (B) x-y view of colored layers in A. Arrowheads denote DSFs and open arrows denote actin arcs. The closed arrow denotes actin on top of the cell body. Intensity levels of each section in A and B were normalized so that structure throughout the cell could be displayed in a single image irrespective of relative intensity. (C) Maximum projection of the ventral actin filaments from below the yellow broken line in B. (D) Maximum projection of dorsal actin filaments from above the dotted line in B. The graph plots the intensity of dorsal actin filaments (green line) against cell height (blue line) along the region from the line in B. Bars, 10 µm.