Table 1.
Values of EC50 to noradrenaline obtained from aortic rings with and without endothelium, in presence of absence of L-NAME, indomethacin or yohimbine from adult male rats exposed or not to chronic stress and ethanol, alone or in combination
| Control EC50 (x10-7 M) | Stress EC50 (x10-7 M) | Ethanol EC50 (x10-7 M) | Stress/ethanol EC50 (x10-7 M) | |
|---|---|---|---|---|
| With endothelium | ||||
| Saline | 3.97± 0.06 (10) | 3.85 ± 0.04 (10) | 4.88 ± 0.06 (10) | 4.29 ± 0.08 (10) |
| L-NAME (10-4 M) | 2.48 ± 0.05 (09) | 2.36 ± 0.05 (09) | 2.60 ± 0.04 (09) | 2.64 ± 0.08 (09) |
| Indomethacin (10-5 M) | 3.85 ± 0.05 (08) | 3.56 ± 0.06 (08) | 5.39 ± 0.05 (08) | 3.58 ± 0.06 (08) |
| Yohimbine (10-6 M) | 10.76 ± 0.06 (09) | 10.19 ± 0.06 (09) | 10.23 ± 0.04 (09) | 9.00 ± 0.06 (09) |
| Without endothelium | ||||
| Saline | 0.61 ± 0.10* (10) | 0.83 ± 0.07* (10) | 0.64 ± 0.06 (10) | 0.67 ± 0.09* (10) |
| L-NAME (10-4 M) | 0.77 ± 0.06* (09) | 1.18 ± 0.11* (09) | 1.10 ± 0.07* (09) | 0.90 ± 0.06* (09) |
| Indomethacin (10-5 M) | 0.83 ± 0.05* (08) | 1.88 ± 0.06* (08) | 1.47 ± 0.05* (08) | 1.30 ± 0.08* (08) |
| Yohimbine (10-6 M) | 1.82 ± 0.05* (09) | 1.94 ± 0.08* (09) | 2.10 ± 0.05* (09) | 2.75 ± 0.07* (09) |
Control: received water “ad libitum”; ethanol: received ethanol solution 20% instead of drinking water; stress: immobilization in a metallic containment tube (1 hour/day, 5 days/week for 6 weeks); chronic stress/ethanol: submitted to immobilization stress and exposed to 20% ethanol. Data expressed as mean ± SEM and, between parentheses, the number of independent determinati ons.
*
p < 0.05 in relation to the respective aorta with endothe lium.