Table I.
Gene symbol | Forward primer | Reverse primer |
---|---|---|
Human | 5′→3′ | 5′→3′ |
GAPDH | GAAGGTGAAGGTCGGAGTC | GAAGATGGTGATGGGATTTC |
IFNA | TGGCAACCAGTTCCAGAAG | GACAACCTCCCAGGCACAA |
HUMAN IFNB1 | TCAGAAGCTtCTGTGGCAAT | TCATGAGTTTTCCCCTGGTG |
IRF7 | TATACCATCTACCTGGGCTTCGG | GCTCCAGCTTTCTGGAGTTCTCATT |
OASL | AGGACTGTTGCTATGACAACAGGGA | TGCTGCTGAGAAGCTGCCTCTC |
ISG15 | TTTGCCAGTACAGGAGCTTGTG | GCTCAGAGGTTCGTCGCATTT |
IFI44 | TTCGATGCGAAGATTCACTG | CCCTTGGAAAACAGACCTCA |
Mouse | 5′→3′ | 5′→3′ |
Gapdh | GGTGCTGAGTATGTCGTGGAGTCTA | AAAGTTGTCATGGATGACCTTGG |
Ifna | TGTCTGATGCAGCAGGTGG | AAGACAGGGCTCTCCAGAC |
Ifnb1 | CACTTGAAGAGCTATTACTGGAGGG | CTCGGACCACCATCCAGG |
Ly6a | GGAGGCAGCAGTTATTGTGGATTCT | GTGGGAACATTGCAGGACCCCA |
OaSl2 | AGCCGTGATGGAGCTCCTCGT | GGATGATGGGCCGGTCTCCCT |
Oasl1 | TGGAGGGTGAAGAGAGCACCCG | AGGCGAGCGTGCAATGGCTT |
IRF7 | TCCGGTACCAGGGTCCAGCC | CGGGAGCGCACACGTGATGT |
Ifng | TGCCAAGTTTGAGGTCAACAACCC | C C ACCCCG AATC AG C AGCG A |
qRT-PCR, quantitative real-time polymerase chain reaction.