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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: Dev Biol. 2012 Aug 10;371(1):23–34. doi: 10.1016/j.ydbio.2012.07.033

Fig. 8. Activation of canonical Wnt pathway overrides the effect of Hh inhibition and restores proliferative signals.

Fig. 8

(A-C) Immunohistochemical analyses of proliferating cells using anti-BrdU (green) sera in control (A), cyclopamine (B) and cyclopamine+BIO (C) treated tissues. (D) Quantitative analysis of the BrdU labeled cells from control, cyclopamine and cyclopamine+BIO treated tissues (n=4; **, p<0.005). (E) Cell cycle kinetics of cells treated with DMSO (white bars), cyclopamine (black bars) and cyclopamine+BIO (grey bars). Note the relative increase in S- and G2/M- phase cells upon activating Wnt signaling in the background of Hh inhibition. Data points represent average cell populations from all experiments (n=3; *, p<0.01; **, p<0.005). Error bars indicate s.e.m.