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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: Dev Biol. 2012 Aug 10;371(1):23–34. doi: 10.1016/j.ydbio.2012.07.033

Fig. 9. Hh signaling modulates Wnt-mediated transcriptional activity.

Fig. 9

(A) qRT-PCR analysis of Wnt3a transcript expression from the regenerating limb tissue at 21d. Expression of Wnt3a mRNA is significantly reduced upon Hh inhibition relative to the controls (n=3; *, p<0.01). (B) Transcriptional assay using TCF-reporter construct in myoblast cells in the presence of Wnt3a alone or in combination with increasing concentrations of cyclopamine. Wnt3a mediated activation of reporter activity is significantly reduced upon inhibition of Hh signaling in a dose dependent manner (n=3; *, p < 0.01). Error bars indicate s.e.m.