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. Author manuscript; available in PMC: 2014 Nov 7.
Published in final edited form as: Cell. 2013 Nov 7;155(4):894–908. doi: 10.1016/j.cell.2013.10.004

Figure 4. Axotomy increases acetylation of histone H3.

Figure 4

(A) Cytoplasmic and nuclear fraction were prepared from axotomized (+Ax) or naive (−Ax) cultured DRG neurons and analyzed by western blot (N, nuclear fraction; C, cytoplasm fraction; H3, histone H3; Ac-H3, acetylated histone H3). (B) DRG were collected 24 hours after sciatic nerve surgery in the presence of vehicle (+V) or 10mM EGTA (+E) and analyzed by western blot. (C) Quantification of (B) (n=3; ***p<0.001; ns, not significant). (D) DRGs collected 24 hours after sciatic nerve axotomy were stained with Ac-H3 and TUJ1. Scale, 50µm. (E) Average intensity of Ac-H3 from (D) and from sections collected from HDAC5 KO mice (n=6 for each; ***p<0.001, *p<0.05). (F) DRG sections stained with HDAC3 and TUJ1. Scale, 50µm. (G) Average of relative intensity of nuclear HDAC3 over cytoplasmic HDAC3 in WT and HDAC5 KO mice (n=13 for each; ***p<0.001, *p<0.05). (H) The RGC layer of retina that received or not a prior (24 hours) optic nerve axotomy were stained for Ac-H3 and DAPI. Scale, 10µm. Data are represented as mean±SEM. See also Figure S1.