Table 2.
Face centered central composite design (FCCCD) for the optimization of phytase production from recombinant E. coli DH5α showing the corresponding experimental and predicted phytase activity (U/mL).
| Run order | Seed age (h) | Inoculums level (%, v/v) | L-arabinose conc. (%, w/v) | Cell conc. at 600 nm | Harvesting time (h) | Phytase activity (U/mL) | |
|---|---|---|---|---|---|---|---|
|
| |||||||
| Experimental | Predicted | ||||||
| 1 | 3 (−1) | 7.5 (+1) | 2 (+1) | 0.7 (+1) | 2.5 (−1) | 533.65 | 662.38 |
| 2 | 7 (0) | 5 (0) | 0.002 (−1) | 0.5 (0) | 10 (0) | 2277.57 | 2433.86 |
| 3 | 7 (0) | 5 (0) | 2 (+1) | 0.5 (0) | 10 (0) | 1031.55 | 696.46 |
| 4 | 3 (−1) | 7.5 (+1) | 0.002 (−1) | 0.3 (−1) | 2.5 (−1) | 571.95 | 833.81 |
| 5 | 3 (−1) | 2.5 (−1) | 0.002 (−1) | 0.7 (+1) | 2.5 (−1) | 1363.48 | 1568.10 |
| 6* | 7 (0) | 5 (0) | 1.001 (0) | 0.5 (0) | 10 (0) | 1657.11 | 1565.16 |
| 7 | 7 (0) | 5 (0) | 1.001 (0) | 0.5 (0) | 17.5 (+1) | 1437.53 | 2049.57 |
| 8* | 7 (0) | 5 (0) | 1.001 (0) | 0.5 (0) | 10 (0) | 1271.56 | 1565.16 |
| 9 | 3 (−1) | 5 (0) | 1.001 (0) | 0.5 (0) | 10 (0) | 1289.43 | 1423.60 |
| 10* | 7 (0) | 5 (0) | 1.001 (0) | 0.5 (0) | 10 (0) | 1953.30 | 1565.16 |
| 11 | 7 (0) | 5 (0) | 1.001 (0) | 0.7 (+1) | 10 (0) | 1271.56 | 1662.90 |
| 12 | 7 (0) | 5 (0) | 1.001 (0) | 0.3 (−1) | 10 (0) | 1835.85 | 1467.43 |
| 13 | 11 (+1) | 7.5 (+1) | 2 (+1) | 0.3 (−1) | 2.5 (−1) | 824.73 | 827.28 |
| 14 | 3 (−1) | 2.5 (−1) | 0.002 (−1) | 0.3 (−1) | 17.5 (+1) | 5017.30 | 4620.39 |
| 15* | 7 (0) | 5 (0) | 1.001 (0) | 0.5 (0) | 10 (0) | 1445.19 | 1565.16 |
| 16 | 7 (0) | 5 (0) | 1.001 (0) | 0.5 (0) | 2.5 (−1) | 194.82 | 249.56 |
| 17 | 11 (+1) | 2.5 (−1) | 0.002 (−1) | 0.7 (+1) | 17.5 (+1) | 4123.63 | 4213.63 |
| 18 | 11 (+1) | 7.5 (+1) | 0.002 (−1) | 0.7 (+1) | 2.5 (−1) | 1833.29 | 1235.18 |
| 19 | 7 (0) | 7.5 (+1) | 1.001 (0) | 0.5 (0) | 10 (0) | 1618.81 | 2205.27 |
| 20* | 7 (0) | 5 (0) | 1.001 (0) | 0.5 (0) | 10 (0) | 1598.39 | 1565.16 |
| 21 | 11 (+1) | 5 (0) | 1.001 (0) | 0.5 (0) | 10 (0) | 2106.50 | 1706.73 |
| 22 | 11 (+1) | 7.5 (+1) | 2 (+1) | 0.7 (+1) | 17.5 (+1) | 1440.08 | 1375.03 |
| 23 | 11 (+1) | 7.5 (+1) | 0.002 (−1) | 0.3 (−1) | 17.5 (+1) | 3916.81 | 4287.46 |
| 24* | 7 (0) | 5 (0) | 1.001 (0) | 0.5 (0) | 10 (0) | 1710.73 | 1565.16 |
| 25 | 3 (−1) | 2.5 (−1) | 2 (+1) | 0.7 (+1) | 17.5 (+1) | 773.66 | 745.35 |
| 26 | 11 (+1) | 2.5 (−1) | 2 (+1) | 0.3 (−1) | 17.5 (+1) | 1613.71 | 1718.38 |
| 27 | 3 (−1) | 7.5 (+1) | 2 (+1) | 0.3 (−1) | 17.5 (+1) | 1013.67 | 819.18 |
| 28 | 3 (−1) | 2.5 (−1) | 2 (+1) | 0.3 (−1) | 2.5 (−1) | 280.87 | 197.60 |
| 29 | 11 (+1) | 2.5 (−1) | 0.002 (−1) | 0.3 (−1) | 2.5 (−1) | 788.98 | 770.40 |
| 30 | 7 (0) | 2.5 (−1) | 1.001 (0) | 0.5 (0) | 10 (0) | 2259.70 | 2340.03 |
| 31 | 11 (+1) | 2.5 (−1) | 2 (+1) | 0.7 (+1) | 2.5 (−1) | 1514.13 | 1561.58 |
| 32 | 3 (−1) | 7.5 (+1) | 0.002 (−1) | 0.7 (+1) | 17.5 (+1) | 4769.63 | 4277.03 |
Note:
*
Central point.