Table 1.
β2-Glycoprotein I antigen isolation procedure: comparison of standard and optimized protocols.
| Standard protocol | Optimized protocol | β2GPI isolation from the same starting material after both protocols | ||
|---|---|---|---|---|
| AB plasma donors (n) | 14 | 6 | 2 | |
| Number of isolations | 12 | 5 | 1 (isolation I) | 1 (isolation II) |
| Starting volume, mean ± SD (mL) | 254 ± 24 | 246 ± 8 | 250 | 255 |
| Number of aliquots | 1 | 3 | 1 | 3 |
| Precipitation duration, mean ± SD (min) | 63 ± 6 | 20 ± 1 | 61.25 | 21.25 |
| Mass of isolated β2GPI, mean ± SD (mg) | 6.56 ± 1.38* | 9.94 ± 1.57* | 7.22 | 10.21 |
| Efficiency improvement | 51.5% | 41.4% | ||
β2GPI, β2-glycoprotein I. *Significantly higher mass (P = 0.004) as compared to standard protocol (Student's t-test, two-tail).