Table 2.

Isolation of human polyclonal high avidity IgG antibodies against β2-glycoprotein I: a representation of isolation efficiency calculation.

Starting material (plasma)	V o (mL)	205
	*C o HAv anti-β2GPI (μg/mL)	33.9
	*m o anti-β2GPI (μg)	6950
1. step	*m start HAv anti-β2GPI (μg)	6950
IgG isolation	*m isolated HAv anti-β2GPI (μg)	6049
(protein G column)	η (1. step)	0.870
2. step	*m start HAv anti-β2GPI (μg)	6049
HAv aβ2GPI isolation	*m isolated HAv anti-β2GPI (μg)	620
(β2GPI column)	η (2. step)	0.102
	η total (%)	8.9%

Concentrations and calculated masses were determined by in-house anti-β2GPI ELISA (*). β2GPI: β2-glycoprotein I; HAv anti-β2GPI: high avidity anti-β2GPI IgG antibodies; V _o: volume of plasma used for isolation; C _o: starting concentrations of HAv anti-β2GPI; m _o, m _start, and m _isolated: calculated masses of HAv anti-β2GPI before isolation and before and after each step; and η: estimated efficiency of isolation.