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Complementation of ΔAtxA mutant and visualization of expression differences. Western blot expression analysis of protective antigen is shown in (a). Lane 1, Ames 35. Lane 2, Ames 35 ΔAtxA. Lane 3, Ames 35 ΔAtxA + pIU68. (b) MochiView images of read counts for the area of pXO1 overlapping the AtxA ORF in representative WT and ΔAtxA samples. The y-axis is normalized to the highest count value in the region and transformed to a log base-2 scale.
