Table 3.
Predicted mRNA binding targets of sRNA-1 and sRNA-2 on pXO1
| Small RNA | Closest ORF (Locus Tag) | Protein name or ID | Region of predicted mRNA | Region of redicted sRNA | Energy of interaction (kJ/mol) | Z-score |
|---|---|---|---|---|---|---|
| sRNA-1 |
pXO1_0199 |
relA-like |
184-196 |
125-137 |
−13.98 |
−2.13 |
| pXO1_0146 |
AtxA |
(-138)-(-129) |
75-84 |
−13.53 |
−2.01 |
|
| pXO1_0113 |
YP_016444.2 |
239-259 |
62-82 |
−12.79 |
−1.82 |
|
| pXO1_0080 |
S-layer domain protein |
637-653 |
116-137 |
−12.47 |
−1.73 |
|
| pXO1_0182 |
Integrase/recombinase |
20-29 |
75-84 |
−12.05 |
−1.62 |
|
| sRNA-2 | pXO1_0066 |
YP_016397.2 |
1079-1103 |
61-86 |
−14.47 |
−3.21 |
| pXO1_0067 |
RepX (FtsZ-like) |
268-278 |
68-78 |
−13.36 |
−2.72 |
|
| pXO1_0010 |
YP_016341.2 |
178-199 |
61-78 |
−13.32 |
−2.7 |
|
| pXO1_0130 |
UDP-glucose 6-dehydrogenase |
1006-1023 |
69-86 |
−12.53 |
−2.35 |
|
| pXO1_0113 | YP_016444.2 | 107-132 | 77-104 | −12.52 | −2.35 |
RNAPredator predictions of the five mRNA targets having the lowest Z-scores for each sRNA are listed. Z-scores quantify the deviation of the binding energy for that interaction from the set of all possible sRNA-mRNA binding interactions.