Table 2.
The estimated LD 50 of Yessotoxin (YTX) in different developmental stages of RSP design
| Design | Dose (μg/kg BW) | Proportion of dead mice |
LD
50
with 95% CI (μg/kg BW) |
|
|---|---|---|---|---|
| Isotonic regression | Spearman-Karber | |||
| Basic RSP |
400 |
3/9 (0.33) |
463 (383 – 535) |
457 (400 – 522) |
| 450 |
4/9 (0.44) |
|||
| 500 |
7/9 (0.78) |
|||
| 600 |
5/9 (0.56) |
|||
| *) Included k-adjustment factor |
400 |
3/9 (0.33) |
481 (362 – 566) |
480 (408 – 565) |
| 462 |
4/9 (0.44) |
|||
| 499 |
5/9 (0.56) |
|||
| 581 |
5/9 (0.56) |
|||
| *) Optimising use of mice |
400 |
1/3 (0.33) |
447 (378 – 504) |
444 (379 – 521) |
| 462 |
5/9 (0.56) |
|||
| 499 |
5/7 (0.71) |
|||
| 581 |
4/5 (0.80) |
|||
| *) Multinomial decision variable with all four design levels | 400 |
1/3 (0.33) |
473 (442 – 517) | 471 (430 – 516) |
| 465 |
3/7 (0.43) |
|||
| 468 |
4/9 (0.44) |
|||
| 482 | 3/5 (0.60) | |||
The results are expressed with 95% Confidence Intervals. *) Simulated data based on the 36 observations in the basic RSP model.