Table 3.

Mean values of estimated LD ₅₀ of Yessotoxin in different pathways using 4-level RSP designs

Pathway	Dose on design level 1	Dose on design level 2	Dose on design level 3	Dose on design level 4	Mean LD50 (μg/kgBW)	95% CI of Mean LD50 (μg/kgBW)
4-Level RSP design with binomial outcome, k-adjustment factor and 9 animals at each design level
01	400 M = 3.29	581 M = 6.23	499 M = 5.01	462 M = 4.38	457	449 – 466
02	400 M = 3.29	581 M = 6.23	499 M = 5.01	536 M = 5.07	513	510 – 516
03	400 M = 3.29	219 M = 0	301 M = 1.05	338 M = 1.97	391	389 – 392
4-Level RSP design with binomial outcome, k-adjustment factor and increasing number of mice with increasing design levels
01	400 M = 1.10	581 M = 3.35	499 M = 3.9	462 M = 4.41	464	449 – 479
02	400 M = 1.10	581 M = 3.35	499 M = 3.9	536 M = 5.57	510	501 – 520
03	400 M = 1.10	219 M = 0	301 M = 0.82	338 M = 1.96	379	374 – 384
4-Level RSP design with multinomial outcome, k-adjustment factor and increasing number of mice with increasing design levels
01	400 M = 1.10	482 M = 2.45	499 M = 3.87	502 M = 5.06	501	498 – 503
02	400 M = 1.10	482 M = 2.45	499 M = 3.87	496 M = 4.97	490	470 – 510
03	400 M = 1.10	482 M = 2.45	465 M = 3.48	468 M = 4.51	470	432 – 508
04	400 M = 1.10	482 M = 2.45	465 M = 3.48	462 M = 4.41	455	353 – 557

The results are expressed with 95% Confidence interval. M represents the mean of dead mice based on 10,000 simulations on the assigned dose.