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. 2014 Mar 25;14:76. doi: 10.1186/1471-2180-14-76

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Copyright © 2014 Ibrahim et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression of CCR5 ligands in liver and spleen tissues from infected mice. Wild type (WT) and CCR5^−/− (KO) mice were infected intraperitoneally with T. gondii tachyzoites. Liver and spleen tissues were collected and their total RNA content was isolated at 0, 3 and 5 days post-infection (dpi). Expression of the target mRNA was determined and compared to expression levels at 0 dpi using quantitative PCR. Bars represent the average for each experimental group (0 dpi, n = 3; 3 dpi, n = 5; 5 dpi, n = 9). RH-GFP (GFP): parasites transfected with GFP alone; RH-OE (OE): parasites transfected with TgCyp18HA and GFP.