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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: J Mol Biol. 2008 May 22;381(2):478–486. doi: 10.1016/j.jmb.2008.05.036

Figure 4.

Figure 4

1H NMR magnetization transfer experiment. The top portion shows the pulse sequence used in the experiment. The middle portion illustrates the route of magnetization transfer, from the water through the protein to the lipid. The lower panel shows the 1H MAS NMR spectrum of rod outer segment disk membranes before (solid line) and immediately after photoactivation of rhodopsin (dashed line) as described below. Immediately after photoactivation a significant increase (up to 11%) in the saturation of lipid proton resonances was observed. Reproducibility of signal intensities was 1%. The effect was recorded as a function of time by recording intensity of the well-resolved =CH-CH2-CH= resonance (2.8 ppm) of polyunsaturated hydrocarbon chains (see insert, and Fig. 4). Attenuation increased also for other resonances, in particular those with contributions to intensity from rhodopsin protons. See Figure S1 in supplemental information for an example difference spectrum.