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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: Nat Genet. 2009 Aug 9;41(9):968–976. doi: 10.1038/ng.428

Figure 2. Generation and characterization of iPS cells derived from mature B and T lymphocytes by four factors.

Figure 2

(a) Sorting strategy of tdTomato+ (gate not shown) mature CD3+ splenic T lymphocytes, immature IgM+ and mature IgMlow IgDhigh B lymphocytes from 10 week-old CD8-iPS chimeras by FACS. (b) ES-like morphology of tdTomato+ Oct4-GFP+ iPS colonies derived from mature B and T cells. (c) (top) Viable newborn chimeras derived from CD3-iPS and IgD-iPS cell lines show red fluorescence originating from the labeled iPS cell lines. Shown are tdTomato+ chimeric pups and non-chimeric littermates (marked by asterisks) under regular light (left) and UV light (right). (bottom) Adult chimeras derived from CD3-T iPS, IgM-B and IgD-B cell iPS show obvious coat color chimerism.