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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: Nat Genet. 2009 Aug 9;41(9):968–976. doi: 10.1038/ng.428

Figure 5. Effect of proliferation rate of hematopoietic cells on reprogramming potential.

Figure 5

(a) Experimental design: HSCs, B and T cells were grown under either proliferation-inducing or – survival-promoting conditions at the time of transgene expression. The formation of iPS colonies was scored 18 days later, 3 days after doxycycline discontinuation. (b) Table summarizing the different growth factor conditions for HSCs, B and T cells, the resultant fraction of cycling cells (measured by Hoechst or PI staining) and efficiency of iPS formation. Note that reprogramming potential correlates with differentiation stage but not with proliferation rate. The TPO concentration differed in the high (20ng/ml) and low (0.5ng/ml) proliferation condition. Efficiencies of HSCs were determined by single-cell sorting into 96-wells into multiple 96-well plates. Efficiencies of B and T cells were assessed by plating 1×10^5 cells into 12-well dishes in duplicates or triplicates.

KL, Kit-ligand; T, TPO; FL, Flt3-ligand; C, CpG; L, LPS; CD3, αCD3 antibody; CD28, αCD28 antibody; 3, IL-3; 6, IL-6; 7, IL-7; 11, IL-11; 15, IL-15.