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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: Science. 2008 Sep 25;322(5903):945–949. doi: 10.1126/science.1162494

Figure 1. Analysis of pluripotency markers in Adeno-iPS cells.

Figure 1

(A) Brightfield (upper panel) and fluorescence (lower panel) images of an Adeno-iPS cell clone established from Sox2-GFP fetal liver cells taken at passage 0 (P0) and passage 2 (P2). (B) Expression of endogenous c-myc, Klf4, Oct4, Sox2 and nanog measured by qPCR in Adeno-iPS cells derived from fetal liver (FL), fibroblasts (TTF) and hepatocytes (HEP) as well as in V6.5 control ES cells. (C) Bisulfite sequencing of the Oct4 and Nanog promoters in hepatocytes, ES cells and iPS cells derived from hepatocytes. Open circles represent unmethylated CpGs; closed circles denote methylated CpGs. (D) Expression levels of endogenous gapdh (G) as well as adenoviral c-myc (M), Klf4 (K), Oct4 (O) and Sox2 (S) in fibroblasts three days after infection with adenoviruses (TTF + 4 adenos), ES cells and Adeno-iPS cells derived from fetal liver, fibroblasts and hepatocytes. Error bars indicate one standard deviation. The absence of a bar indicates that the respective cDNA was not detected.

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