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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Biomater Sci. 2014 Feb 7;2(5):655–665. doi: 10.1039/C3BM60274E

Figure 4. HT1080 cell fate in AHA hydrogels with varying stiffnesses in atmospheric and hypoxic conditions.

Figure 4

(A) Proliferation of HT1080 cells encapsulated in stiff hydrogels over the three-day experiment in atmospheric and hypoxic conditions. (B) Representative cell cycle flow analysis of HT1080 cells encapsulated in stiff hydrogels over the three-day experiment in atmospheric and hypoxic conditions. (C) Quantification of the percentage of apoptotic cells (sub-G1) in the various conditions. (Di, ii) Quantitative RT-PCR for BNIP3 and GLUT-1 expression in HT1080 cells encapsulated in hydrogels of medium stiffness (3D) and in Petri dishes (plates) in atmospheric and hypoxic conditions. Significance levels (n=3) were set at: *p<0.05, **p<0.01, and ***p<0.001.