Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Mar 24;13:72. doi: 10.1186/1476-4598-13-72

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Harris et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

siRNA-mediated inhibition of WEE1 decreases medulloblastoma cell proliferation. (A) Daoy and UW228 cells transfected with a siRNA targeting WEE1 (siWEE1) displayed a decrease in cell proliferation when compared to a non-silencing siRNA (siRNA N.C.). Forty-eight hours after transfection cells were seeded into E-plates and real-time cell proliferation monitored using the xCELLigence system. (B) Transfection with siWEE1 significantly decreased the relative colony number compared to siRNA N.C. Representative pictures for each treatment group are above the quantifying bar graphs. (C) RNAi-mediated inhibition of WEE1 decreased the levels of WEE1 protein in both the Daoy and UW228 cell lines 72 hours after transfection.