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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: Neurobiol Dis. 2012 Aug 4;48(3):526–532. doi: 10.1016/j.nbd.2012.07.024

Fig. 1.

Fig. 1

ATXN1 affects histone acetylation. a) The level of histone acetylation in cerebellar lysates of SCA1 mice is compared to wild-type littermates by staining with an antibody specific to acetylated histone H3. Staining with an actin-specific antibody serves as a loading control. The intensity of the lanes is quantified by densitometry where the histograms show the mean value of densitometry with error bars showing SE. n=9 pairs of adult mice. p=0.0347 using paired two-tailed t-test. b) Histone acetylation is decreased in cells expressing mutant ATXN1. HeLa cells transiently transfected with mutant ATXN1 84Q-GFP or wild-type ATXN1 2Q-GFP were sorted for GFP positive (transfected) and GFP negative (untransfected) cells. Western blot with an anti-GFP antibody shows the efficiency of sorting. Western blot with an anti-actin antibody serves as a loading control. Data is representative of three independent experiments. p=0.0122 for ATXN1 84Q (with asterisk) and p=0.3471 for ATXN1 2Q using paired two tailed t-test.