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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: Neurobiol Dis. 2012 Aug 4;48(3):526–532. doi: 10.1016/j.nbd.2012.07.024

Fig. 2.

Fig. 2

Synergistic transcriptional repression by ATXN1 and LANP. a) N2A cells were co-transfected with the CBP-Gal4 (100 ng/well) and increasing amounts (25, 50 and 100 ng) of LANP and ATXN1 2Q or 84Q-GFP to cause progressive repression of CBP activity when compared to cells transfected with CBP-Gal4 and empty vector. ANOVA p<0.05 comparing 50 or 100 ng of each construct (LANP, ATXN1 2Q or ATXN1 84Q) to CBP control. No statistical difference (p>0.05) was present when comparing repression caused by equal DNA amounts of ATXN1 2Q and ATXN 1 84Q. b) N2A cells were co-transfected with CBP-Gal4 and low levels (5 ng) of LANP and ATXN1 (2Q or 84Q) that by themselves do not cause any inhibition. When co-transfected LANP and ATXN1 84Q synergistically inhibit CBP activity (asterisk signifies p<0.05; Analysis of variance (ANOVA) with post-hoc t test comparing ATXN1 84Q to ATXN1 84Q and LANP). ATXN1 2Q did not show statistical synergism (p>0.05). The data in a. and b. is representative of three (a) or eight (b) independent experiments, Error bars=SE.