Skip to main content
. Author manuscript; available in PMC: 2015 Mar 10.
Published in final edited form as: Brain Res. 2014 Jan 16;1551:45–58. doi: 10.1016/j.brainres.2014.01.013

Figure 3. p38 MAPK knockout attenuates astrogliosis in primary astrocyte cultures.

Figure 3

A. Depicts are representative Western blot of primary astrocyte cultures derived from wild type (WT) and GFAP/p38 knock out (KO) mice. Loss of p38 MAPK expression was associated with a marked reduction GFAP expression in primary astrocyte derived from GFAP/p38 knockout mice. B. Depicts are representative Western blots of primary astrocyte cultures subjected to normoxia or OGD/reoxygenation insult. Lower GFAP expression was observed in primary astrocyte culture derived from GFAP/p38 knockout mice under normoxic condition and subjected to OGD/reoxygenation insult. C. Quantitative analysis of GFAP Western blots demonstrated that p38 MAPK knockout significantly decreased OGD/reoxygenation induced increase of GFAP expression in primary astrocyte cultures. * p < 0.05 vs. normoxia wild type. # p < 0.05 vs. hypoxia wild type D. Depict is a representative gelatin zymograph of media from wild type and GFAP/p38 knockout astrocyte cultures. E. Quantitative analysis of gelatin zymography demonstrated that p38 knockout significantly reduced MMP-9 activity under normoxia and after OGD/reoxygenation insult. ***p < 0.001 vs. normoxia wild type.. * p < 0.05 vs. normoxia wild type. ### p < 0.001 vs. hypoxia wild type.