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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Kidney Int. 2013 Nov 27;85(4):972–980. doi: 10.1038/ki.2013.463

Figure 1. Generation of podocyte-specific stochastic multicolor reporter mice.

Figure 1

(A) The experimental approach for the generation of podocyte specific multicolor reporter mice. The brainbow-2.1 construct encoding four fluorescent proteins driven by the strong CAG promoter was inserted into the Rosa26 locus. Upon Cre recombination, the Neomycin (Neo) cassette is removed and the multicolor construct recombines randomly to result in four possible outcomes with different fluorescent proteins being expressed. Representative 2-D images of a glomerular cross-section from a healthy six-week old male (B) NPHS2Cre/R26R-ConfettiTG/WT (C) NPHS2Cre/R26R-ConfettiTG/TG mice, showing randomly labeled podocyte cell bodies (red or yellow), podocyte cell nuclei (green) or podocyte cell membranes (blue). (D) Representative z-stack projection reconstruction of all colors from NPHS2Cre/R26R-ConfettiTG/TG mouse. (E) Representative 3-D volume (Volocity-based) reconstruction of all four color channels of the NPHS2Cre /R26R-ConfettiTG/WT mice.