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. 2014 Apr 15;9(4):e93634. doi: 10.1371/journal.pone.0093634

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© 2014 Abreu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Animals were euthanized on day 21 post infection (p.i.), perfused, and the kidneys were collected for histologic analysis. Collagen deposition in the renal cortex was visualized using Picrosirius Red. Representative photomicrographs of (A) control_21d, (B) control+BSA_21d, (C) control+treated+BSA_21d, (D) infected+treated_21d, and (E) infected+treated+BSA_21d (n = 6 per group). Bar = 20 µm. Collagen deposition is quantified in (F). Values are expressed as a percentage of collagen deposition per area (means ± standard error). *Statistically significant compared with control_21d. #Statistically significant compared with infected+treated _21d (P<0.05).