Table 1. ATHB17Δ113 can bind both Class II and Class I DNA targets and ATHB17Δ113 containing V182A-Q185A-N186A mutation cannot bind Class II DNA target in in vitro assay (measured by Surface Plasmon Resonance (SPR).
| Protein | Sequence | kon(1/Ms)×105 | koff(1/s)×10−4 | KD(nM) | Relative KD |
| ATHB17Δ113 | CAGACAAT C ATTGCGGC (Class II) | 42.3±16.0 | 880.6±416 | 20.4±3.3 | 1.0 |
| ATHB17Δ113 | CAGACAAT T ATTGCGGC (Class I) | 29.0±16.4 | 1012.7±387 | 37.7±14.0 | 1.8 |
| ATHB17Δ113 | CAGCTCAGTCTGAGGC (non-consensus) | - | - | NB | - |
| ATHB17Δ113 (V182A-Q185A-N186A) | CAGACAAT C ATTGCGGC (Class II) | - | - | NB | - |
Binding affinities and Kinetic constants of ATHB17Δ113 interacting with Class I and Class II type DNA, measured by Biacore 2000, globally fitted. SPR measurements with Biacore 2000 were at 25°C in HBS-EP, 100 ug/ml BSA (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% Tween-20,100 ug/ml BSA). Equilibrium dissociation constant KD = koff/kon.