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. 2014 Apr 15;9(4):e94173. doi: 10.1371/journal.pone.0094173

Figure 1. Generation of Pou4f1 and Pou4f2 conditional knockout alleles.

Figure 1

(A) Targeting strategy for generating Pou4f1 conditional null allele. (B) Targeting strategy for generating Pou4f2 conditional null allele. (C) Southern blot confirmed selection of Pou4f1cko allele and PCR genotyping of Pou4f1loxP heterozygous and homozygous. (D) Southern blot confirmed selection of Pou4f2cko allele and PCR genotyping of Pou4f2loxP heterozygous and homozygous. White boxes are the non-coding exon sequences and black boxes are the coding sequences. Thick bars are the sequences used to generate the homologous arms in the targeting vector. Restriction enzyme sites: A, AvrII; B, BamHI; E, EcoRI; H, HindIII; N, NotI; S, SacII; X, XbaI.