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. 2014 Apr 15;9(4):e94857. doi: 10.1371/journal.pone.0094857

Table 1. Production, or not, of sclerotia by Aspergillus niger strain IBT 29019, IBT 24631, and IBT 24634, and production of apolar indoloterpenes on Czapek yeast autolysate agar with or without added plant parts.

CYA + additional fruit/cereal grain Aspergillus niger strain Sclerotia produced 10,23-dihydro-24,25-dehydroaflavinine produced Number of apolar indoloterpenes detected by liquid chromatography diode array detection (LC-DAD)
None IBT 29019 -* - 0/0
None IBT 24631 - - 0/0
None IBT 24634 - - 0/0
Raisin (CYAR) IBT 29019 ++* + 2/2/2/4 **
Raisin (CYAR) IBT 24631 +* + 1/1/1/2 **
Raisin (CYAR) IBT 24634 - - 0/0
Mulberry IBT 29019 ++ + 2
Mulberry IBT 24631 - - 0
Mulberry IBT 24634 - - 0
Blueberry IBT 29019 ++ + 2/3
Blueberry IBT 24631 - - 0
Blueberry IBT 24634 - - 0
Cranberry IBT 29019 ++ + 2/7
Cranberry IBT 24631 - - 0
Cranberry IBT 24634 - - 0
Goji berry IBT 29019, 24631, 24634 - - 0
Apricot IBT 29109 ++ + 2/7
Apricot IBT 24631 −/+*** + 0/1
Apricot IBT 24634 - - 0/0
Prune IBT 29019 ++ + 2/3
Prune IBT 24631 −/+*** + 0/1
Prune IBT 24634 - - 0/0
Mango, 40% IBT 29019 ++ + 3/5
Mango, 40% IBT 24631, 24634 - - 0/0
Mango peel IBT 29019 ++ + 2
Papaya, 40% & 4% IBT 29019 - - 0/0
Corn IBT 29019 - - 0
Corn IBT 24631 + + 1
Corn IBT 24634 - - 0
White rice, 50% IBT 29019 ++ + 11/12
White rice, 50% IBT 24631 + + 2
White rice, 50% or 40% IBT 24634 - - 0
Brown rice, 50% IBT 29019 ++ + 13/13
Brown rice, 40% IBT 29019 + + 2/1
Brown rice, 50% IBT 24631 + + 1
Brown rice, 50% IBT 24634 - - 0

*-: no sclerotia produced, +: few scattered sclerotia, ++: many sclerotia (>10) surrounding each piece of fruit, or directly on the rice or corn.

**More indoloterpenes were detected using mass spectrometric detection than diode array detection in this case where both methods were compared. In IBT 29019 on CYAR, 4 recognizable indoloterpenes were detected using LC-mass spectrometric detection (in bold), but only two according to DAD.

***−/+: sclerotia not produced in one experiment, but produced in the other

The conidia were frozen at −18°C for three weeks before inoculation.