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WT, ptpn11a-/-, ptpn11b-/- and ptpn11a-/- ptpn11b-/- embryos were lyzed and immunoblotted with phosho-specific antibody for ERK and AKT; membranes were then reprobed for total Erk and Akt expression to control for loading. The blots were quantified and the ratio of phosphorylated protein/total protein from three independent experiments is indicated below each lane.
