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. 2014 Apr 15;9(4):e94746. doi: 10.1371/journal.pone.0094746

Figure 4. Tissue culture replication of CV-A16 viruses.

Figure 4

Viral replication was monitored by the induction of CPE by the prototype CV-A16-G10 and the circulating Changchun CV-A16 strains. Viruses were isolated from infected Vero cells. Viral titers were determined as previously described (Ref). Equal amounts of virus were used to infect Vero, SK-N-SH, RD and L929 cells. Viral replication resulting in CPE of >90% of cells was recorded as+(A). (B) Representative CPE caused by the prototype CV-A16-G10 and Changchun circulating CV-A16 viruses are shown. Images were obtained using phase contrast microscopy (Olympus IX51, Center Valley, PA).