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. 2013 Oct 1;2(4):e26548. doi: 10.4161/worm.26548

Table 2. Thirty-two validated GLD-1 targets have 38 GLD-1 binding regions.

     GLD-1 Targets*      Microarray        GLD-1 binding
     Sequence name      Gene name      FLAG/IgG†      P-value‡      DescriptionΠ      5′ end      3′ end
     ZC513.6      oma-2      13.47      2.00E-05      Zinc finger protein of the TIS11 finger type      Y      Y
     T05G5.7      rmd-1      8.75      1.10E-03      TPR domain      N/D      Y
     T11F8.3      rme-2      8.36      8.00E-05      Yolk receptor      Y      Y
     Y75B12B.1      N/D      8.01      8.00E-04      Probable transposase      Y      N
     C50B6.2      nasp-2      7.66      6.00E-04      Histone binding protein      Y      N
     C07G2.1      cpg-1      7.40      2.00E-04      Chitin binding peritrophin      N      Y
     F32B6.5      sss-1      6.10      1.01E-02      Sperm-specific protein family, S class      Y      Y
     F44D12.4      N/D      5.11      5.90E-03      PDZ domain      N/D      N
     H02I12.1      cbd-1      4.87      2.00E-04      Chitin binding peritrophin      Y      N
     R09B3.1      exo-3      4.83      1.10E-03      Exonuclease      Y      N
     T01H8.1      rskn-1      4.69      1.00E-03      Protein kinase C      N      Y
     C09G9.6      oma-1      4.17      0.00E+00      Zinc finger protein of the TIS11 finger type      Y      Y
     ZK829.5      tbx-36      4.11      5.50E-03      T-box transcription factor      N      Y
     F14B4.2      N/D      4.04      2.62E-02      Hexokinase      N      Y
     T23G11.2      gna-2      3.99      3.00E-04      Glucosamine phosphate N-Acetyl transferase      Y      N
     W09C5.2      unc-59      3.90      4.20E-03      Septin required for axon and DTC migration      N      Y
     T07A9.6      daf-18      3.84      7.00E-04      PTEN lipid phosphatase      N/D      N
     F43G6.1      dna-2      3.68      5.10E-03      Endonuclease/helicase      N      Y
     T01C3.2      N/D      3.38      1.46E-02      unknown      N/D      Y
     F26D10.10      gln-5      3.32      1.00E-04      Glutamine sythetase      N      Y
     T27E9.3      cdk-5      3.13      8.30E-03      Cyclin-dependent kinase      N/D      Y
     Y49E10.14      pie-1      2.90      6.30E-03      CCCH zinc finger protein      N      Y
     F19B6.2      ufd-1      2.55      3.60E-03      Ubiquitin fusion degradation protein      N      Y
     Y47G6A.8      crn-1      2.55      3.00E-03      Cell death-related nuclease      N      Y
     T13F2.9      N/D      2.53      2.06E-02      unknown      N/D      Y
     M04F3.1      rpa-2      2.37      1.00E-03      unknown      Y      N
     T27F2.3      bir-1      2.21      2.70E-03      Chromosome segregation      N/D      Y
     F35B12.5      sas-5      2.16      1.00E-04      Centriole formation      Y      Y
     T06E6.2      cyb-3      1.86      7.10E-03      A member of the cyclin B family      N      Y
     F58A4.3      hcp-3      1.80      5.60E-03      Centromere protein (CENP)-A homolog      N      Y
     F52B5.5      cep-1      1.64      1.94E-02      Ortholog of the human tumor suppressor p52      N      Y
     Y73B6A.5§      lin-45      N/D      N/D      Ortholog of the vertebrate protein RAF      N      Y
*

Sequence and gene names of 32 targets examined; †FLAG/IgG ratios in decreasing order in microarray analysis. With the exception of T06E6.2, F58A4.3, and F52B5.5, all targets have an enrichment of > 2.0 in the GLD-1-FLAG IP compared with the IgG IP; ‡P values of microarray analysis; §Y73B6.A.5 was not spotted in the microarray analysis but was identified previously (Lee and Schedl, 2001); ΠShort descriptions of the functions of targets if known; (Y) and (N) indicate that GLD-1 was detectable (binding) and undetectable (not binding) via western blot analysis after biotin-RNA pull down assays. (N/D) indicate that binding assays were not performed.