Table 1. Kinetic parameters and substrate inhibition of CtCBM22A_GOOOX-VN on cello-oligosaccharides and xylo-oligosaccharides.

	Kinetic parameters*			Substrate inhibition
	k cat (min−1)	K m (mM)	k cat/K m (mM−1 min−1)	V i/V max (%)	K i (mM)	n H **
Glucose	1,090 ± 16	11 ± 1	95 ± 7	–	–	–
Cellobiose	1,148 ± 5	0.060 ± 0.001	19,300 ± 300	45	2.9 ± 0.3	1.3
Cellotriose	1,210 ± 60	0.09 ± 0.01	12,800 ± 1,900	50	1.88 ± 0.16	1.5
Cellopentaose	1,080 ± 30	0.08 ± 0.01	14,100 ± 1,800	56	2.2 ± 0.3	1.5
Cellohexaose	1,190 ± 40	0.13 ± 0.01	9,000 ± 1,000	62	1.4 ± 0.3	1.3
Xylose	1,230 ± 20	135 ± 8	9 ± 1	–	–	–
Xylobiose	1,350 ± 30	0.09 ± 0.01	14,500 ± 1,400	75	8 ± 2	1.5
Xylotriose	1,330 ± 30	0.12 ± 0.01	11,000 ± 1,000	71	6 ± 3	1.2
Xylopentaose	1,630 ± 50	0.10± 0.01	15,700 ± 1,900	–	–	–
Xylohexaose	1,340 ± 40	0.12 ± 0.02	11,500 ± 1,600	–	–	–

^*Data are mean values ± standard errors; 16 nM of enzyme was used in each reaction.

^**Hill coefficient, values greater that one indicate positive cooperative binding.

– No inhibition detected with used substrate concentrations.