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. 2014 Apr 15;9(4):e95171. doi: 10.1371/journal.pone.0095171

Figure 4. rAMF increases the in vitro chemotaxis of MSCs towards HCC and their adhesion to endothelial cells.

Figure 4

A) Pretreatment of BM-MSCs with 1 µg/ml rAMF (black bars) increases chemotaxis towards TCM derived from HuH7 or HC-PT-5 cells compared to untreated cells (white bars). B) Wound-healing assay of MSCs after pretreatment with rAMF or control (DMEM). Representative images were taken 24 hours after scratching. C) Adhesion to HMEC-1 endothelial cells was increased in BM-MSCs exposed to rAMF. D) Expression of AMF receptor (AMFR), GDP dissociation inhibitor 2 (GDI-1), caveolin-1 (CAV-1) and caveolin-2 (CAV-2) by qRT-PCR. *p<0.05, **p<0.01 and ***p<0.001 vs untreated cells (DMEM, white bars, unpaired Student's t-test). E) AMFR expression was increased in AMF-treated MSCs evaluated by western blot.