Table 2. Kinetics and affinity analysis of enkephalin and glycosylated enkephalins with ASGPR.# .
| Kon (1/M.s) | Koff (1/s) | KD (µM) | |
| Enkephalin | 349.7±69.8 | 0.0970±0.0290 | 277.4±96.0 |
| Gal-Enk | 328.3±84.8 | 0.0720±0.0250 | 219.3±164.0 |
| Lac-Enk | 40.7±6.3 | 0.0086±0.002 | 211.3±70.9 |
| Gal-Lac-Enk | 45.0±28.9 | 0.0041±0.003 | 91.1±22.1 |
| D-Galactose | ND | ND | 59.7±30.1 |
| D-Lactose | ND | ND | 19.2±3.28 |
#
ND: Kinetics values for D-galactose and D-lactose could not be uniquely determined due to size differences between the analyte (ASGPR, 37 kDa) and the ligand (D-galactose and D-lactose 180 and 342 Da, respectively) resulting in small RU values and larger than acceptable Chi2 values for the kinetic analysis. Calculation of affinity was not affected.